Nipah Virus Rna -

Nipah Virus Sequences from Humans and Bats during Nipah.

Nipah virus RNA detected by real-time reverse-transcription polymerase chain reaction in blood, oropharyngeal swab, urine, and semen against days post onset of illness. Abbreviations: Ct, cycle threshold; NiV, Nipah virus; OP, oropharyngeal. Nipah virus is a single stranded RNA virus and a member of the paramyxovirus family and the Henipavirus genus. The Henipavirus genus includes two of the most lethal viruses known to man: Hendra and Nipah. The natural reservoir of the virus is the fruit bat 2. The incubation time from exposure to symptom onset is around 9 days 6. Nipah virus NiV was first reported from Malaysia in 1999. Additional NiV outbreaks have occurred in Bangladesh 2–4 and India 5,6. NiV is a negative-sense enveloped RNA encoding for 6 genes nucleocapsid, phosphoprotein, matrix, fusion protein, glycoprotein, and polymerase 7,8.

Nipah virus is an enveloped, non-segmented, negative-strand RNA virus very closely related to Hendra virus, the virus is named after the Malaysian village where it was first discovered. A member of the genus henipavirus, a new class of virus in the Paramyxoviridae family. Sep 15, 2004 · Nipah virus contains a single-stranded RNA of about 18,000 nucleotides associated with the viral proteins of the replicative complex the nucleoprotein N, the phosphoprotein P, and the polymerase L enclosed by a lipid bilayer envelope containing the attachment protein G and the fusion protein F Chua et al., 2000, Wang et al., 2001, Chan et al., 2001. Of the 12 laboratory-confirmed cases, 10 showed NiV RNA in oral swab specimens. We obtained surface swab samples for 6 Nipah patients; 5 had evidence of NiV RNA on >1 surface: 4 patients contaminated towels, 3 bed sheets, and 1 the bed rail. Patients with NiV RNA in oral swab samples were significantly more likely than other Nipah patients to die. May 30, 2018 · Nipah virus RNA has been detected in semen from an individual who received treatment for Nipah virus infection, with RNA detected at day 26. May 26, 2018 · Nipah virus can then enter the bloodstream and disseminate throughout the host in either free form or by binding host leukocytes. Nipah virus has been shown to bind to CD3 leukocytes without entry or replication of the virus Other target organ of Nipah virus the brain, spleen and kidneys.

Specific detection of Nipah virus using real-time RT-PCR.

Nipah virus NiV is a member of the family Paramyxoviridae, genus Henipavirus.NiV was initially isolated and identified in 1999 during an outbreak of encephalitis and respiratory illness among pig farmers and people with close contact with pigs in Malaysia and Singapore. The virus uses its own RNA replicase, also known as RNA-dependent RNA polymerase RdRp, to form positive RNA template strands through complementary base pairing. The positive RNA acts as mRNA, which is translated into structural capsomere proteins and viral RdRp by the host's ribosomes. Creating a vaccine is currently extremely difficult because the mutation rate of RNA viruses is extremely high as is most zoonotic diseases, of which Nipah virus is both 9. However several studies have been conducted in targeting the F and G proteins which would inhibit binding of the NiV cells to any host cell 9. Nipah virus infections have killed 72–86% of the infected individuals in Bangladesh and India. The infections are spread via bodily secretions of bats, pigs and other infected individuals. Even though, the disease was first detected in the human population in 1998, there are. Surveillance for Nipah virus NV was conducted in Thailand's bat population. Immunoglobulin G antibodies to NV were detected with enzyme immunoassay in 82 of 1,304 bats. NV RNA was found in bat saliva and urine. These data suggest the persistence of NV infection in Thai bats.

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